Physical map location of the peptide methionine sulfoxide reductase gene on the Escherichia coli chromosome

Physical map location of the peptide methionine sulfoxide reductase gene on the Escherichia coli chromosome.

The enzyme peptide methionine sulfoxide reductase (MsrA), the product of the msrA gene, catalyzes the reduction of methionine sulfoxide residues in proteins to methionine (1, 3, 4). During aerobic metabolism, methionine in proteins may be oxidized to methionine sulfoxide by biological reagents such as hydrogen peroxide, hydroxyl radicals, and hypochlorite and superoxide ions (reviewed in refere...

Location of gyrA on the physical map of the Escherichia coli chromosome.

Methionine sulfoxide reduction and assimilation in Escherichia coli: new role for the biotin sulfoxide reductase BisC.

Methionine ranks among the amino acids most sensitive to oxidation, which converts it to a racemic mixture of methionine-S-sulfoxide (Met-S-SO) and methionine-R-sulfoxide (Met-R-SO). The methionine sulfoxide reductases MsrA and MsrB reduce free and protein-bound MetSO, MsrA being specific for Met-S-SO and MsrB for Met-R-SO. In the present study, we report that an Escherichia coli metB1 auxotrop...

Location of the fpg gene on the Escherichia coli chromosome.

The fpg gene of E. coli specifies a DNA glycosylase that excises a ring-opened form of N7-methylguanine (2-6-diamino-4hydroxy-5-N-methylformamidopyrimidine [Fapy]) from the chromosome. This gene has been isolated, on plasmids that overproduce the Fapy-DNA glycosylase activity, and sequenced; the protein has been purified to physical homogeneity (1, 2). However, no fpg mutants are known and the ...

Location of the endA gene coding for endonuclease I on the physical map of the Escherichia coli K-12 chromosome.

The endonuclease I of Escherichia coli is an RNA-inhibitable DNase which is located in the periplasmic space of the E. coli cell (3); it cleaves native DNA into oligonucleotides (7). It is assumed that in the noninhibited, RNA-free state the enzyme introduces nucleotide sequence-independent doublestrand breaks into DNA (2, 9), whereas the inhibited, RNAcomplexed enzyme probably causes only nick...